現(xiàn)貨供應(yīng),歡迎訂購(gòu): : 胰酶 產(chǎn)品信息: Crystalline Trypsin Solution,貨號(hào) : 03-047-1A/B,胰酶 Cell Dissociation Solution(Non-Enzymatic),貨號(hào): 03-071-1B,(71號(hào)) 71號(hào)細(xì)胞分離試劑是BI公司*開發(fā)的不含任何酵素、蛋白水解酶及動(dòng)物組分的一種試劑。運(yùn)用離子螯合技術(shù)使貼壁細(xì)胞溫和的脫 落,zui大程度地保留完整的細(xì)胞表面蛋白及其生物功能,長(zhǎng)時(shí)間使用也無(wú)需擔(dān)心細(xì)胞受損。可適用于血清及不含血清培養(yǎng)細(xì)胞,貼壁更緊密的細(xì)胞可選用木瓜蛋白酶 或結(jié)晶。 Papain Dissociation Solution,貨號(hào) : 03-072-1B, 水晶對(duì)細(xì)胞損傷較小、細(xì)胞存活率高,較長(zhǎng)時(shí)間的使用也無(wú)需擔(dān)心。 木瓜蛋白酶與相比有更廣泛的特異性,更廣泛的降解蛋白物質(zhì),效率更高,對(duì)細(xì)胞、組織造成的損害更小,極少負(fù)面的影響,同時(shí)避免了動(dòng)物成分的污染。 的使用方法: 1. 使用不含鈣和鎂的PBS溶液沖洗細(xì)胞 2. 每一個(gè)T-25培養(yǎng)瓶中加1ml的木瓜蛋白酶分離溶液 3. 15-30°C孵育,當(dāng)細(xì)胞*分離,快速將細(xì)胞懸浮在5-10ml生長(zhǎng)培養(yǎng)基中。 4. 離心細(xì)胞,去掉上清 更多相關(guān)產(chǎn)品: BIOLOGICAL INDUSTRIES 05-010-1A "DCCM-1 without L-Glutamine 哺乳動(dòng)物細(xì)胞懸浮培養(yǎng)無(wú)血清培養(yǎng)基" 500ml 4℃ BI公司 05-015-1A "DCCM-2 without L-Glutamine 哺乳動(dòng)物細(xì)胞懸浮培養(yǎng)無(wú)血清培養(yǎng)基" 500ml 4℃ BI公司 05-040-1A "Low Protein Media BSA-Free (LPM), without L-Glutamine 哺乳動(dòng)物細(xì)胞懸浮培養(yǎng)無(wú)血清培養(yǎng)基" 500ml 4℃ BioInd公司 01-015-5A Basal Medium Eagle (Earle's) 10X 500ml AMB BioInd公司 01-340-1B MEM Non-Essential Amino Acids Solution, 100X Conc. 100ml 4℃ BioInd公司 02-010-1A Earle's Balanced Salt Solution 500ml AMB BioInd公司 02-010-5A Earle's Balanced Salt Solution, 10X Conc., without Sodium Bicarbonate 500ml AMB BIOLOGICAL INDUSTRIES 02-010-5A Earle's Balanced Salt Solution, 10X Conc., without Sodium Bicarbonate 500ml AMB BIOLOGICAL INDUSTRIES 02-011-5B Earle's Balanced Salt Solution, 10X Conc., without Phenol Red, without Sodium Bicarbonate 100ml AMB BIOLOGICAL INDUSTRIES 02-015-1B Hanks' Balanced Salt Solution 100ml AMB BIOLOGICAL INDUSTRIES 02-016-1A Hanks' Balanced Salt Solution, without Phenol Red 500ml AMB BIOLOGICAL INDUSTRIES 02-017-1A Hanks' Balanced Salt Solution, without Calcium and Magnesium 500ml AMB BIOLOGICAL INDUSTRIES 02-017-1B Hanks' Balanced Salt Solution, without Calcium and Magnesium 100ml AMB BI公司 02-018-1A Hanks' Balanced Salt Solution, without Calcium and Magnesium, without Phenol Red 500ml AMB BI公司 02-020-1A Dulbecco's Phosphate Buffered Saline (DPBS) 500ml 4℃ BI公司 02-020-1B Dulbecco's Phosphate Buffered Saline (DPBS) 100ml 4℃ BI公司 02-023-1A Dulbecco's Phosphate Buffered Saline (DPBS), without Calcium and Magnesium 500ml AMB BI公司 02-023-1B Dulbecco's Phosphate Buffered Saline (DPBS), without Calcium and Magnesium 100ml AMB BioInd公司 02-023-5A Dulbecco's Phosphate Buffered Saline (DPBS), 10X Conc., without Calcium and Magnesium 500ml AMB BioInd公司 02-045-1A Alsever's Solution 500ml AMB BIOLOGICAL INDUSTRIES 02-045-1B Alsever's Solution 100ml AMB BIOLOGICAL INDUSTRIES 03-010-1B "Bovine Albumin Solution (10%), Fraction V in saline 牛白蛋白(10%)" 100ml -20℃ BIOLOGICAL INDUSTRIES 03-015-1B Ethylenediaminetetraacetic Acid (EDTA) Disodium Salt Solution, 0.05% in DPBS 100ml AMB BIOLOGICAL INDUSTRIES 03-020-1A "L-Glutamine Solution, 29.2mg/ml in Saline 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