Anti-ATP5O [4C11C10D12] antibody (ab110276)抗ATP5O [4C11C10D12]抗體產品信息： - Product nameAnti-ATP5O [4C11C10D12] antibodySee all ATP5O primary antibodies ...
- Description
Mouse monoclonal [4C11C10D12] to ATP5O - Tested applicationsICC/IF, WB, Flow Cyt, IHC-P more details
- Species reactivity
Reacts with: Cow, Human, African Green Monkey - Immunogen
Recombinant mature Human ATP5O. - Positive control
- Isolated mitochondria from Human heart and Bovine heart, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), Human colon tissue, HL-60 cells
Properties Applications Our Abpromise guarantee covers the use of ab110276 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. | Application | Notes |
|---|
| ICC/IF | ICC/IF: Use a concentration of 0.5 - 1 µg/ml. heat-induced antigen-retrieval improves signal | | WB | WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 23 kDa. | | Flow Cyt | Flow Cyt: Use a concentration of 1 µg/ml. | | IHC-P | IHC-P: 1/500. |
Target - FunctionMitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
- Sequence similaritiesBelongs to the ATPase delta chain family.
- Cellular localizationMitochondrion. Mitochondrion inner membrane.
- Information by UniProt
Database links - Entrez Gene: ATP5O Cow
- Entrez Gene: 539 Human
- Omim: 600828 Human
- SwissProt: P13621 Cow
- SwissProt: P48047 Human
- Unigene: 49172 Cow
- Unigene: 409140 Human
Alternative names - ATP synthase O subunit mitochondrial precursor antibody
- ATP synthase subunit O antibody
- ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit antibody
Anti-ATP5O [4C11C10D12] antibody images ![Western blot - ATP5O antibody [4C11C10D12] (ab110276)](http://a.abcam.com/ps/datasheet/images/110/ab110276/ATP5O-Primary-antibodies-ab110276-1.jpg) Western blot - ATP5O antibody [4C11C10D12] (ab110276) All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 0.5 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Predicted band size : 23 kDa ![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276)](http://a.abcam.com/ps/datasheet/images/110/ab110276/ATP5O-Primary-antibodies-ab110276-5.jpg) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATP5O antibody [4C11C10D12] (ab110276) Human colon tissue fixed with 4% PFA and antibody detection with ab110276 using the ABC system. ab110276 diluted 1/500 and incubated for 1 hour. Sections were incubated in peroxidase-conjugated rabbit anti-mouse immunoglobulins (diluted 1/100 in 4% BSA in PBST) for 1 hour at room temp. ![Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276)](http://a.abcam.com/ps/datasheet/images/110/ab110276/ATP5O-Primary-antibodies-ab110276-6.jpg) Immunocytochemistry/ Immunofluorescence - ATP5O antibody [4C11C10D12] (ab110276) Mitochondrial localization of ATP5O. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110276 (0.5 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG1 isotype specific secondary antibody (2 µg/ml). ![Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276)](http://a.abcam.com/ps/datasheet/images/110/ab110276/ATP5O-Primary-antibodies-ab110276-4.jpg) Flow Cytometry - ATP5O antibody [4C11C10D12] (ab110276) HL-60 cells were stained with 1 µg/mL ab110276 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry. ![Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276)](http://a.abcam.com/ps/products/110/ab110276/Images/ab110276-172336-ATP5OPrimaryantibodiesab1102767.jpg) Western blot - Anti-ATP5O antibody [4C11C10D12] (ab110276) All lanes : Anti-ATP5O [4C11C10D12] antibody (ab110276) at 1 µg/ml
Lane 1 : HDFn (human) cell lysates at 20 µg
Lane 2 : COS7 (monkey) cell lysates at 20 µg
Lane 3 : H4IIE (rat) cell lysates at 20 µg
Lane 4 : MEF (mouse) cell lysates at 20 µg
Lane 5 : bovine heart mitochondria lysates at 5 µg
Predicted band size : 23 kDaSecondary antibody: Goat-Anti-Mouse-IR800 1:4000
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Polyglutamylated100ul4274Tubulin抗體一抗 Abcamab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulin抗體一抗 Abcamab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptin抗體一抗 Abcam ab11414Mouse monoclonal [SN6] to CD105100ug3996CD105hystemcell一抗 Abcam ab11415Mouse monoclonal [SN6] to CD105 （FITC）100ug5029CD105hystemcell一抗 Abcam ab11417Mouse monoclonal [D9D10] to Interferon gamma （FITC）50ug3996IFN-gammahystemcell一抗 Abcam ab11418Mouse monoclonal [Z-ZE4] to IGFBP150ug3996IGFBP1hystemcell一抗 Abcam ab11419Mouse monoclonal [9C8] to GADD15350ug4418GADD153hystemcell一抗 Abcam ab1142Rabbit polyclonal to Bcl10100ul3996Bcl-1hystemcell一抗 Abcam ab11420Rabbit polyclonal to ERp2950ul3996ERp29hystemcell一抗 Abcam ab11421Rabbit polyclonal to ERp5750ul3996ERp57hystemcell一抗 Abcam ab11422Rabbit polyclonal to ERp7250ug3996ERp72hystemcell一抗 Abcam ab11423Mouse monoclonal [1234] to Rapsyn50ug3996Rapsynhystemcell一抗 Abcam ab11424Rabbit polyclonal to EDG150ug3996EDG1hystemcell一抗 Abcam ab11425Mouse monoclonal [1351] to Syntrophin50ug3996Syntrophinhystemcell一抗 Abcam ab11426Rabbit polyclonal to Calbindin - Neuronal Marker50ug3996Calbindinhystemcell一抗 Abcam ab11427Rabbit polyclonal to Parvalbumin50ug3996Parvalbuminhystemcell一抗 Abcam ab11428Rabbit polyclonal to S100 alpha50ug3996S100 alphahystemcell一抗 Abcam ab11429Rabbit polyclonal to RELM beta50ul3996RELM betahystemcell一抗 Abcam ab1143Rabbit polyclonal to Acinus100ul3996Acinushystemcell一抗 Abcam ab11430Rabbit polyclonal to Resistin50ul3996Resistinhystemcell一抗 Abcam ab11431Rabbit polyclonal to Resistin50ug3996Resistinhystemcell一抗 Abcam ab11432Rabbit polyclonal to PDIA650ul3996P5hystemcell一抗 Abcam ab11433Mouse monoclonal [5] to VCP50ul4418VCPhystemcell一抗 Abcam ab11434Rabbit polyclonal to Ataxin 750ug3996Ataxin 7hystemcell一抗 Abcam ab11436Rabbit polyclonal to PSMD750ug3996Proteasome 19S S12hystemcell一抗 Abcam ab11437Rabbit polyclonal to Proteasome 20S alpha 550ug3996Proteasome 20S alpha 5hystemcell一抗 Abcam ab11438Mouse monoclonal [LZA2] to Listeria monocytogenes250ug3996Listeria monocytogeneshystemcell一抗 Abcam ab11439Mouse monoclonal [LZH1] to Listeria monocytogenes250ug3996Listeria monocytogeneshystemcell一抗 Abcam ab1144Rabbit polyclonal to Acinus100ul3996Acinushystemcell一抗 Abcam ab11441Mouse monoclonal [LX32] to Listeria monocytogenes250ug3996Listeria monocytogeneshystemcell一抗 Abcam ab11442Mouse monoclonal [HBe7] to Hepatitis B Virus E Antigen250ug3996HBeAghystemcell一抗 Abcam ab11443Mouse monoclonal [HBe3] to Hepatitis B Virus E Antigen （HRP）200ug3996HBeAghystemcell一抗 Abcam ab11444Mouse monoclonal [HBe8 ] to Hepatitis B Virus E Antigen500ug3996HBeAghystemcell一抗 Abcam ab11447Mouse monoclonal [B-G5 ] to IL2 （FITC）50ug3996IL-2hystemcell一抗 Abcam ab11448Rat monoclonal [MQ1-17H12] to IL2250ug3996IL-2hystemcell一抗 Abcam ab11449Mouse monoclonal [B-E8 ] to IL6100ug3996IL-6hystemcell一抗 Abcam ab1145Rabbit polyclonal to FLASH100ul3996FLASHhystemcell一抗 Abcam ab11450Mouse monoclonal [B-K8 ] to IL8100ug3996IL-8hystemcell一抗 Abcam ab11451Mouse monoclonal [4D5] to IP10250ug3996IP-10hystemcell一抗 Abcam ab11453Mouse monoclonal [h.mcp.3] to MCP3250ug3996MCP-3hystemcell一抗 Abcam ab11456Mouse monoclonal [1E8-269] to TGF alpha1ml3996TGF alphahystemcell一抗 Abcam ab11457Mouse monoclonal [B-D9] to TNF alpha （FITC）50ug3996TNF-alphahystemcell一抗 Abcam ab1132Mouse monoclonal [1718] to Thyroid Hormone Receptor100ul3996Thyroid Hormone Receptorhystemcell一抗 Abcam ab11320Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulinhystemcell一抗 Abcam ab11321Rabbit polyclonal to gamma Tubulin - Centrosome Marker100ul3996gamma Tubulinhystemcell一抗 Abcam ab11322Mouse monoclonal [TUB-11] to epsilon Tubulin100ul3996epsilon Tubulinhystemcell一抗 Abcam ab11323Mouse monoclonal [6-11B-1] to Tubulin （acetyl K40）100ul3996Tubulinhystemcell一抗 Abcam ab11324Mouse monoclonal [B3] to Tubulin - Polyglutamylated100ul4274Tubulinhystemcell一抗 Abcam ab11325Mouse monoclonal [TUB-1A2] to Tubulin100ul3996Tubulinhystemcell一抗 Abcam ab11326Mouse monoclonal [100/2] to alpha Adaptin100ul3996alpha Adaptinhystemcell一抗 Abcam ab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptinhystemcell一抗 Abcam ab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptinhystemcell一抗 Abcam ab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18hystemcell一抗 Abcam ab1133Rabbit polyclonal to GABARAP100ul3996GABARAPhystemcell一抗 Abcam ab11330Mouse monoclonal [C6G9] to Carcino Embryonic Antigen CEA200ul3996CEAhystemcell一抗 Abcam ab11331Mouse monoclonal [TD.1] to Clathrin heavy chain100ul3996Clathrin heavy chainhystemcell一抗 Abcam ab11333Mouse monoclonal [1B10] to Fibroblast Surface Protein 200ul3996Fibroblast Surface Proteinhystemcell一抗 Abcam ab11334Mouse monoclonal [LN-5] to Macrophage200ul3996Macrophagehystemcell一抗 Abcam ab11335Mouse monoclonal [45M1] to Gastric Mucin 200ul3996Mucin Gastrichystemcell一抗 Abcam ab11336Rabbit polyclonal to Parvin alpha50ul3996Parvin alphahystemcell一抗 Abcam ab11337Rabbit polyclonal to l Afadin100ul3996l Afadinhystemcell一抗 Abcam ab11338Rabbit polyclonal to l+S Afadin 100ul3996l/S Afadinhystemcell一抗 Abcam ab11339Mouse monoclonal [FA-5] to N Cadherin100ul3996N Cadherinhystemcell一抗 Abcam ab1134Rabbit polyclonal to GABA B Receptor 2100ul3996GABA B Receptor 2hystemcell一抗 Abcam ab11340Mouse monoclonal [GC-4] to N Cadherin100ul3996N Cadherinhystemcell一抗 Abcam ab11342Mouse monoclonal [CH-19] to pan Cadherin - Plasma Membrane Marker （FITC）100ul3996Cadherin panhystemcell一抗 Abcam ab11343Rabbit polyclonal to CASK100ul3996CASKhystemcell一抗 Abcam ab11346Rabbit polyclonal to alpha 1 Catenin100ul3885alpha 1 Cateninhystemcell一抗 Abcam ab11347Rabbit polyclonal to alpha 2 Catenin100ul3996alpha 2 Cateninhystemcell一抗 Abcam ab11348Mouse monoclonal [15B8] to beta Catenin （Cy3 ®）100ul3996beta Cateninhystemcell一抗 Abcam ab11349Mouse monoclonal [BC-76] to beta Catenin （phospho S33）50ul3996beta Cateninhystemcell一抗 Abcam ab1135Rabbit polyclonal to GABA B Receptor 1100ul3996GABA B Receptor 1hystemcell一抗 Abcam ab11350Mouse monoclonal [BC-22] to beta Catenin （phospho S33+S37）50ul3996beta Cateninhystemcell一抗 Abcam ab11351Rabbit polyclonal to delta 2 Catenin100ul3996delta 2 Cateninhystemcell一抗 Abcam ab11352Rabbit polyclonal to delta 2 Catenin100ul3996delta 2 Cateninhystemcell一抗 Abcam ab11354Mouse monoclonal [4C3] to HMGB150ug3996HMGB1hystemcell一抗 Abcam ab11355Mouse monoclonal [ICRF44] to CD11b50tests3996CD11bhystemcell一抗 Abcam ab11356Mouse monoclonal [ICRF44] to CD11b （FITC）50tests3996CD11bhystemcell一抗 Abcam ab11358Mouse monoclonal [A3D8] to CD4450tests3996CD44hystemcell一抗 Abcam ab11359Mouse monoclonal [8.4A6] to ICAM1100ul3996CD54,ICAM1hystemcell一抗 Abcam ab1136Rabbit polyclonal to Artemin100ul3996Arteminhystemcell一抗 Abcam ab11364Mouse monoclonal [FMC46] to CD62L100ul3996CD62Lhystemcell一抗 Abcam ab11365Mouse monoclonal [FMC-46] to CD62L （FITC）50tests3996CD62Lhystemcell一抗 Abcam ab11366Mouse monoclonal [CXN-32] to Connexin 32/GJB150ul3996Connexin 32,GJB1hystemcell一抗 Abcam ab11367Rabbit polyclonal to Connexin 32/GJB1100ul3996Connexin 32,GJB1hystemcell一抗 Abcamab11327Mouse monoclonal [100/1] to AP1+2 beta1 00ul3996beta 1/2 Adaptin抗體一抗 Abcamab11328Mouse monoclonal [100/3] to gamma Adaptin100ul3996gamma Adaptin抗體一抗 Abcamab11329Mouse monoclonal [AP180-I] to AP180100ul3996AP18抗體一抗 Abcamab1133Rabbit polyclonal to GABARAP100ul3996GABARAP抗體一抗 更多詳情，請： 重慶市華雅干細胞技術有限公司 |
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