熱烈祝賀華雅生物成為TAKARA Cellartis®干細胞研究產(chǎn)品全國代理商 作為一款創(chuàng)新型的產(chǎn)品，Cellartis DEF-CS培養(yǎng)系統(tǒng)使得單細胞干細胞操作成為常規(guī)的操作。該產(chǎn)品是專門針對人類誘導干細胞（hiPS）和人類胚胎干細胞（hES）研發(fā)的高效率增殖用培養(yǎng)基產(chǎn)品體系。該產(chǎn)品系統(tǒng)為all-in-one型，包括了所有組分，客戶不需要另外求購組分。該產(chǎn)品是成分確定的培養(yǎng)基產(chǎn)品，而且不需要飼養(yǎng)層。DEF-CS既可以實現(xiàn)單細胞培養(yǎng)，也可以用于傳統(tǒng)的iPS培養(yǎng)模式以及大規(guī)模干細胞增殖。在使用DEF-CS培養(yǎng)基增殖干細胞時，幾乎沒有背景分化的問題，這使得細胞篩選不再必需。作為一款創(chuàng)新產(chǎn)品，利用DEF-CS培養(yǎng)干細胞時，可以使用酶消化法（enzymatic passaging）實現(xiàn)需要單細胞操作，這一特點十分有利于高通量細胞鑒別篩選（high-throughput screening）、轉染（transfection）、框架接種（scaffold seeding）等。 | ■ 產(chǎn)品特點 | · Feeder-free and chemically defined culture system for efficient expansion of human iPS cells
· Ideal system for culturing iPS cells; cells maintain pluripotency and long-term potential for self-renewal
and differentiation
· Complete kit includes 500 ml basal medium, additives, and coating compound
· Robust system provides high reproducibility and stable growth
· Maintains cells in an undifferentiated state with virtually no background differentiation | ■ 產(chǎn)品應用 | · Scale-up and mass production of human iPS cells
· Single-cell culture of human iPS cells
· Transfection and reprogramming
· High-throughput screening
· Tissue engineering (seeding cells on a scaffold) | | | | Code No. | Product | Size | | Y30012 | Cellartis® DEF-CS™ 500 COAT-1 | 4 mL | | Y30010 | Cellartis® DEF-CS™ 500 Culture System | 1 Kit | | Y30016 | Cellartis® DEF-CS™ 500 Additives | 1 Kit |
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Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems. |
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