| Neural Stem Cell Culture Media | RHB-A和RHB-Basal是兩款無血清、成分*確定的培養(yǎng)基，用于人類或小鼠神經(jīng)干細胞（NS）的定向分化獲取、維持培養(yǎng)、擴增、誘導(dǎo)分化成神經(jīng)細胞等用途。RHB-A可用來培養(yǎng)擴增貼壁的人或小鼠的神經(jīng)干細胞。在RHB-A中培養(yǎng)的神經(jīng)干細胞保持著穩(wěn)定的神經(jīng)干細胞分化發(fā)育成為成熟的神經(jīng)細胞的能力 (1-3) 。另外，RHB-A還用來定向分化小鼠胚胎干細胞(mouse ES)至神經(jīng)前體細胞 (neural precursors) (4-6)。
RHB‐Basal不包含生長因子或是神經(jīng)細胞相關(guān)的添加物。因此，可以RHB‐Basal為基礎(chǔ)，通過添加客戶認為需要的添加物來開發(fā)適合客戶的特殊類型細胞的培養(yǎng)基。 | | | | ■ 產(chǎn)品特點 | · RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent
human and mouse NS cells
· RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be
customized by the addition of supplements | | | | ■ 產(chǎn)品應(yīng)用 | · Derivation of mouse and human NS cells from ES cells and fetal and adult tissues
· Maintenance and propagation of adherent mouse and human NS cells
· Differentiation of mouse and human NS cells into functional neurons
· Differentiation of mouse ES cells to neuronal precursors
· Refer to the Data Sheet for additional examples of use | | | ■ 產(chǎn)品詳情 |  | | Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP). | | | | 參考文獻： | 1. Ying QL, et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture.
Nature Biotechnology 21:183-186.
2. Conti L, et al. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell.
PLoS Biology 3(9):e283.
3. Pollard SM, et al. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex 16:112-120.
4. Diogo MM, et al. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells.
Biotechnology and Applied Biochemistry 49:105-112.
5. Pollard SM, et al. (2008) Fibroblast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors
and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience 38:393:403.
6. Sun Y, et al. (2008) Long-term tripotent differentiation capacity of human neural stem (NS)cells in adherent culture.
Molecular and Cellular Neuroscience 38:245-258.
7. Pollard SM, et al. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are
suitable for chemical and genetic screens. Cell Stem Cell 4:568-580.
8. Abranches E, et al. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis
in the embryo. PLoS ONE 4(7): e6286.
9. Fernandes et al. (2010) Hypoxia enhances proliferation of mouse embryonic stem cell-derived neural stem cells.
Biotechnology and Bioengineering 106: 260–270.
10. Fernandes, T.G., et al. (2010) Different stages of pluripotency determine distinct patterns of proliferation, metabolism, |
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