重慶市華雅干細(xì)胞技術(shù)有限公司 , : 華雅集團(tuán)旗下授權(quán)代理TTAKARA Cellartis干細(xì)胞產(chǎn)品 圖片為展示效果。實(shí)際產(chǎn)品為細(xì)胞株+培養(yǎng)基套裝 人iPS誘導(dǎo)多能干細(xì)胞 (Human Induced Pluripotent Stem Cells) 產(chǎn)品英文名稱:Cellartis® Human iPS Cell Lines 產(chǎn)品中文名稱:Cellartis® 人iPS誘導(dǎo)多能干細(xì)胞 Cellartis人iPS細(xì)胞(Human iPS Cell Line)產(chǎn)品是達(dá)到了工業(yè)客戶使用標(biāo)準(zhǔn)生產(chǎn)的干細(xì)胞產(chǎn)品。該產(chǎn)品包括細(xì)胞和用于增殖培養(yǎng)的Cellartis DEF-CS Culture System培養(yǎng)體系。iPS細(xì)胞已經(jīng)在制品化時(shí)完成了對(duì)DEF-CS培養(yǎng)體系的適應(yīng)。Cellartis DEF-CS Culture System培養(yǎng)體系是組分明確而且不依賴飼養(yǎng)層的便捷的干細(xì)胞培養(yǎng)體系。Cellartis DEF-CS Culture System適合高效率地增殖培養(yǎng)干細(xì)胞,并且保持干細(xì)胞在增殖過(guò)程中幾乎無(wú)分化地生長(zhǎng),這使得不需要在大規(guī)模增殖培養(yǎng)過(guò)程中進(jìn)行細(xì)胞篩選。因此,Cellartis人iPS細(xì)胞(Human iPS Cell Line)產(chǎn)品是一套完整的產(chǎn)品體系,對(duì)于科研級(jí)別和工業(yè)規(guī)模的需求都適合。 Cellartis人iPS誘導(dǎo)多能干細(xì)胞特點(diǎn) ☆細(xì)胞系通過(guò)使用缺陷型多順?lè)醋幽孓D(zhuǎn)錄病毒(defective polycistronic retrovirus)技術(shù)重編程人皮膚成纖維細(xì)胞以轉(zhuǎn)入Oct-4,SOX2,KLF4和c-Myc ☆細(xì)胞系的純度和干細(xì)胞特征經(jīng)過(guò)廣泛分析,包括解凍后的恢復(fù),支原體和細(xì)菌檢測(cè),干細(xì)胞特異性標(biāo)記物的表達(dá)(Oct-4,NANOG,SSEA-3,SSEA-4,TRA-1 -60,TRA-1-81),不表達(dá)分化標(biāo)記(β-3微管蛋白,F(xiàn)OXA2,ASMA)和預(yù)期核型 ☆用Cellartis DEF-CS培養(yǎng)系統(tǒng)優(yōu)化培養(yǎng),試劑盒包括Cellartis DEF-CS 100培養(yǎng)系統(tǒng) ☆適用于所有主要應(yīng)用; 細(xì)胞在分化時(shí)可以分化形成所有三個(gè)胚層的衍生物 Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver Oct-4, SOX2, KLF4, and c-Myc Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (Oct-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected Karyotype Optimized for culture with the Cellartis DEF-CS Culture System Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated Kits include the Cellartis DEF-CS 100 Culture System Cellartis 人iPS誘導(dǎo)多能干細(xì)胞參數(shù) 不同貨號(hào)對(duì)應(yīng)供體年齡性別冷凍代數(shù)和已確認(rèn)分化能力 iPS誘導(dǎo)多能干細(xì)胞的應(yīng)用 細(xì)胞治療、藥物監(jiān)測(cè)和疾病模型 Cellartis 人iPS誘導(dǎo)多能干細(xì)胞組份 不同供體的iPS細(xì)胞系和DEF-CS 100*培養(yǎng)基 *培養(yǎng)基包含基礎(chǔ)培養(yǎng)基、添加劑和包被劑。 保存:細(xì)胞 ≤ –150°C,添加劑 –20°C,基礎(chǔ)培養(yǎng)基和包被劑4 °C。 A. Cellartis Human iPS Cell Lines Cellartis human iPS cell line P11025 (Cat. No. Y00180, not sold separay; sold as a part of Cat.No. Y00185) Cellartis human iPS cell line P11032 (Cat. No. Y00220, not sold separay; sold as a part of Cat.No. Y00225) Cellartis human iPS cell line 4 (ChiPSC4) (Cat. No. Y00260, not sold separay; sold as a part of Cat. No. Y00265) Cellartis human iPS cell line 7 (ChiPSC7) (Cat. No. Y00270, not sold separay; sold as a part of Cat. No. Y00275) Cellartis human iPS cell line 12 (ChiPSC12) (Cat. No. Y00280, not sold separay; sold as a part of Cat. No. Y00285) Cellartis human iPS cell line 18 (ChiPSC18) (Cat. No. Y00300, not sold separay; sold as a part of Cat. No. Y00305) Cellartis human iPS cell line 21 (ChiPSC21) (Cat. No. Y00310, not sold separay; sold as a part of Cat. No. Y00315) Cellartis human iPS cell line 22 (ChiPSC22) (Cat. No. Y00320, not sold separay; sold as a part of Cat. No. Y00325) B. Cellartis DEF-CS 100 Culture System · Cellartis DEF-CS 100 Culture System (Cat. No. Y30020, not sold separay; sold as a part of Cat.Nos. Y00185, Y00225, Y00265, Y00275, Y00285, Y00305, Y00305, and Y00315. A larger 500ml version of this product is sold as Cat. No. Y00310.) o DEF-CS Basal Medium (100 ml) o DEF-CS COAT-1 (for 100 ml) (800 µl) o DEF-CS GF-1 (for 100 ml) (300 µl) o DEF-CS GF-2 (for 100 ml) (100 µl) o DEF-CS GF-3 (for 100 ml) (40 µl) 另行準(zhǔn)備材料:PBS+TrypLE 選擇酶+TC處理ps表面培養(yǎng)器皿 The following materials are required but not supplied: · PBS Dulbecco's with Ca2+ & Mg2+ (D-PBS +/+) · PBS Dulbecco's without Ca2+ or Mg2+ (D-PBS –/–) · TrypLE Select Enzyme (1X), no phenol red · Cell culture vessels, Tissue culture treated polystyrene surface · General cell culture equipment used in cell culture laboratory 參考文獻(xiàn): 1. de Peppo GM, et al. Osteogenic potential of human mesenchymal stem cells and human embryonic stem cell-derived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A.2010 Nov;16(11):3413-3426. 2. Both SK, et al. Differential bone-forming capacity of osteogenic cells from either embryonic stem cells or bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med.2011 Mar;5(3):180-190. 3. de Peppo GM, et al. Free-form-fabricated commercially pure Ti and Ti6A14V Porous Scaffolds support the growth of human embryonic stem cell-derived mesodermal progenitors. ScientificWorldJournal. 2012;2012:646417.Epub 2012 Jan 4. 4. Ou Li, et al. Human embryonic stem cell-derived mesenchymal stroma cells(hES-MSCs) engraft in vivo and support hematopoiesis without suppression immune function: implications for off –the shelf ES-MSC therapies. PLOS ONE.2013; 8(1):e55319. Cellartis 人iPS誘導(dǎo)多能干細(xì)胞訂購(gòu)信息 不同貨號(hào)的區(qū)別:iPS細(xì)胞供體不同 品牌 | Code No. | 包裝量 | 價(jià)格 | Cellartis | Y00185 | 1 Kit | 22718 元 | Cellartis | Y00275 | 1 Kit | 22718 元 | Cellartis | Y00285 | 1 kit | 22718 元 | Cellartis | Y00315 | 1 Kit | 22718 元 | Cellartis | Y00325 | 1 kit | 22718 元 | Cellartis | Y00305 | 1 Kit | 22718 元 |
?華雅集團(tuán)旗下授權(quán)代理TTAKARA Cellartis干細(xì)胞產(chǎn)品,Cellartis的多能細(xì)胞培養(yǎng)系統(tǒng)包括iPS誘導(dǎo)多能干細(xì)胞株、iPS誘導(dǎo)多能干細(xì)胞和ES胚胎干細(xì)胞培養(yǎng)基,iPS基因編輯用單細(xì)胞克隆培養(yǎng)基和多能細(xì)胞檢測(cè)用單克隆抗體。 重慶市華雅干細(xì)胞技術(shù)有限公司以“專注干細(xì)胞研究,提升干細(xì)胞品質(zhì)”為發(fā)展理念,專業(yè)從事干細(xì)胞基礎(chǔ)研究、干細(xì)胞再生醫(yī)學(xué)臨床應(yīng)用研究及干細(xì)胞技術(shù)服務(wù),自主研發(fā)產(chǎn)品熱賣中:人間充質(zhì)干細(xì)胞無(wú)血清培養(yǎng)基 , : |